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Commit 6ccac861 authored by Thulani Hewavithana (qnm481)'s avatar Thulani Hewavithana (qnm481)
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Update README.md

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...@@ -291,20 +291,27 @@ of small blocks, small synteny blocks are incorporated into the ...@@ -291,20 +291,27 @@ of small blocks, small synteny blocks are incorporated into the
corresponding super-synteny blocks, taking into account the subgenome corresponding super-synteny blocks, taking into account the subgenome
placement of the super-synteny blocks as a reference. placement of the super-synteny blocks as a reference.
\- Usage No read in files Three input parameters: ws1, ws2 and ws3. \- Usage No read in files or parameters.
Note: You need to select the most suitable window size parameters for
subgenome1, subgenome2 and subgenome3.
\- Output The execution of SyntenyLink\_sb outputs number of output \- Output The execution of SyntenyLink\_sb outputs number of output
files: abc\_synteny\_chromosome\_names.success.colinear.xlsx, files: modified\_chr\_names{k+1}.xlsx, subgenome\_placement\_blocks.all{k+1}.xlsx, subgenome\_placement\_blocks.all.xlsx. k represents the corresponding subgenome number.
subgenome\_placement\_blocks.all.xlsx and
subgenome\_placement\_blocks.all\_sub{k}.xlsx; There will be m number of
files. k represents the corresponding subgenome number.
The abc\_synteny\_chromosome\_names.success.colinear.xlsx file holds the The modified\_chr\_names{k+1}.xlsx file holds the
replaced gene id\'s with there corresponding chromosome names which they replaced gene id\'s with there corresponding chromosome names which they
bellong to: bellong to if they are not already placed inside the subgenome columns (to detect the genes that has been removed based on density threshold):
Gene_id N1 N1.r N2 N2.r N3 N3.r N4 N4.r N5 N5.r N6 N6.r N7 N7.r
AT1G01900 0 0 0 0 0 0 0 0 0 1 0 0 0 0
AT1G01910 0 0 0 0 0 0 0 0 0 0 0 1 0 0
AT1G01920 0 0 0 0 0 0 0 0 0 0 0 1 0 0
AT1G01930 0 0 0 0 0 0 0 0 0 0 0 1 0 0
AT1G01940 0 0 0 0 0 0 0 0 0 0 0 1 0 0
AT1G01950 0 0 0 0 0 0 0 0 0 0 N6 0 0 0
AT1G01960 0 0 0 0 0 0 0 0 0 0 0 1 0 0
AT1G01970 0 0 0 0 0 0 0 0 0 1 0 0 0 0
The subgenome\_placement\_blocks.all{k+1}.xlsx file holds the placement
of genes in subgenomes optimal for each subgenome considering the subgenome separation optimal for each subgenome in step 4:
Row start # Row end # subgenome1 subgenome2 subgenome3 Row start # Row end # subgenome1 subgenome2 subgenome3
0 123 N10.r N9 N8.r 0 123 N10.r N9 N8.r
...@@ -319,12 +326,6 @@ of genes in subgenomes in step 5: ...@@ -319,12 +326,6 @@ of genes in subgenomes in step 5:
124 698 N10 N8.r N9.r 124 698 N10 N8.r N9.r
699 1029 N6 N9.r N8.r 699 1029 N6 N9.r N8.r
The subgenome\_placement\_blocks.all\_sub{k}.xlsx file holds the optimal placement of genes in subgenomes for each subgenome. Ex: subgenome\_placement\_blocks.all\_sub1.xlsx::
: Row start \# Row end \# subgenome1 subgenome2 subgenome3 0 75 N10.r
N9 N8.r 76 78 N10.r N9.r N8.r 79 123 N10.r N9 N8.r 124 698 N10 N9.r
N8.r
SyntenyLink\_mn SyntenyLink\_mn
--------------- ---------------
...@@ -414,5 +415,3 @@ placemnet accuracy of each subgenome: ...@@ -414,5 +415,3 @@ placemnet accuracy of each subgenome:
Exact match number for subgenome3: 5635 Exact match number for subgenome3: 5635
Missing genes for subgenome3: 2995 Missing genes for subgenome3: 2995
Example
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