From 6ccac86107fce514c905ecffb1eee6fb846a0ea2 Mon Sep 17 00:00:00 2001 From: "Thulani Hewavithana (qnm481)" <qnm481@mail.usask.ca> Date: Fri, 18 Aug 2023 17:52:41 -0600 Subject: [PATCH] Update README.md --- README.md | 35 +++++++++++++++++------------------ 1 file changed, 17 insertions(+), 18 deletions(-) diff --git a/README.md b/README.md index d4a9664..7839f52 100644 --- a/README.md +++ b/README.md @@ -291,20 +291,27 @@ of small blocks, small synteny blocks are incorporated into the corresponding super-synteny blocks, taking into account the subgenome placement of the super-synteny blocks as a reference. -\- Usage No read in files Three input parameters: ws1, ws2 and ws3. - -Note: You need to select the most suitable window size parameters for -subgenome1, subgenome2 and subgenome3. +\- Usage No read in files or parameters. \- Output The execution of SyntenyLink\_sb outputs number of output -files: abc\_synteny\_chromosome\_names.success.colinear.xlsx, -subgenome\_placement\_blocks.all.xlsx and -subgenome\_placement\_blocks.all\_sub{k}.xlsx; There will be m number of -files. k represents the corresponding subgenome number. +files: modified\_chr\_names{k+1}.xlsx, subgenome\_placement\_blocks.all{k+1}.xlsx, subgenome\_placement\_blocks.all.xlsx. k represents the corresponding subgenome number. -The abc\_synteny\_chromosome\_names.success.colinear.xlsx file holds the +The modified\_chr\_names{k+1}.xlsx file holds the replaced gene id\'s with there corresponding chromosome names which they -bellong to: +bellong to if they are not already placed inside the subgenome columns (to detect the genes that has been removed based on density threshold): + +Gene_id N1 N1.r N2 N2.r N3 N3.r N4 N4.r N5 N5.r N6 N6.r N7 N7.r +AT1G01900 0 0 0 0 0 0 0 0 0 1 0 0 0 0 +AT1G01910 0 0 0 0 0 0 0 0 0 0 0 1 0 0 +AT1G01920 0 0 0 0 0 0 0 0 0 0 0 1 0 0 +AT1G01930 0 0 0 0 0 0 0 0 0 0 0 1 0 0 +AT1G01940 0 0 0 0 0 0 0 0 0 0 0 1 0 0 +AT1G01950 0 0 0 0 0 0 0 0 0 0 N6 0 0 0 +AT1G01960 0 0 0 0 0 0 0 0 0 0 0 1 0 0 +AT1G01970 0 0 0 0 0 0 0 0 0 1 0 0 0 0 + +The subgenome\_placement\_blocks.all{k+1}.xlsx file holds the placement +of genes in subgenomes optimal for each subgenome considering the subgenome separation optimal for each subgenome in step 4: Row start # Row end # subgenome1 subgenome2 subgenome3 0 123 N10.r N9 N8.r @@ -319,12 +326,6 @@ of genes in subgenomes in step 5: 124 698 N10 N8.r N9.r 699 1029 N6 N9.r N8.r -The subgenome\_placement\_blocks.all\_sub{k}.xlsx file holds the optimal placement of genes in subgenomes for each subgenome. Ex: subgenome\_placement\_blocks.all\_sub1.xlsx:: - -: Row start \# Row end \# subgenome1 subgenome2 subgenome3 0 75 N10.r - N9 N8.r 76 78 N10.r N9.r N8.r 79 123 N10.r N9 N8.r 124 698 N10 N9.r - N8.r - SyntenyLink\_mn --------------- @@ -414,5 +415,3 @@ placemnet accuracy of each subgenome: Exact match number for subgenome3: 5635 Missing genes for subgenome3: 2995 -Example -======= -- GitLab